2-Chlorotrityl Resin (CTC Resin)

2-Chlorotrityl chloride resin is an acid labile resin for peptide synthesis using Fmoc amino acids. The steric bulk and mild acid conditions for cleavage make 2-chlorotrityl resins useful in many applications. The steric bulk of the 2-chlorotrityl resin prevents diketopiperazide formation during the attachment of the first two Fmoc amino acids, a serious problem in the synthesis of Fmoc-Pro C-terminal and Fmoc-Pro-Xxx C-terminal peptides. Additionally, Fmoc-amino acids can be attached to 2-chlorotrityl chloride resin with essentially no racemization. Amino acid 2-chlorotrityl resins can be alternatives to Fmoc-amino acid Wang resins where racemization of the first Fmoc-amino acid is common as with Cys and His. Since peptide products can be cleaved from the resin with very mild acid treatment, 2-chlorotrityl chloride resin can be used to prepare protected peptide fragments for fragment condensation synthesis of large peptides and proteins.

2-Chlorotrityl chloride resin is moisture sensitive. It must be stored in a tightly sealed container or dessicator. If 2-chlorotrityl chloride resin is stored at low temperatures, the container must be allowed to warm to room temperature before opening. Depending on the size of the container and the amount of 2-chlorotrityl chloride resin, this could take up to two hours. For long-term storage, the 2-chlorotrityl chloride resin container should be flushed with nitrogen or argon before sealing, then the 2-chlorotrityl chloride resin should be stored at -20 °C.

2-Chlorotrityl chloride resin and a large selection of amino acid substituted 2-chlorotrityl resins are available from AAPPTec in catalog and bulk quantities. For a bulk quotation on 2-chlorotrityl chloride resins, please send an email to sales@aapptec.com.  For a complete list of AAPPTec peptide synthesis chemicals, click here.  

Loading 2 Chlorotrityl Chloride Resin (CTC Resin)

  1. Use 1.0 equivalent of the first amino acid. If a lower substitution resin is required, reduce the amount of amino acid. Dissolve the Fmoc-amino acid in DCM (approximately 10 mL per gram of resin). If the Fmoc-amino acid does not dissolve in completely, add a small amount of DMF. (Fmoc-Asn and Fmoc-Gln are not soluble in DCM. Dissolve these amino acids in DMF then add DCM until the volume of the solution is approximately 10 mL per gram. Add a small amount of DMF if needed to obtain a clear solution.)
  2. Add the amino acid solution to the 2-chlorotrityl chloride resin. Add 5.0 equivalent (respective to the amino acid) of DIPEA. Agitate the mixture with a shaker for 5 minutes, then add 1.5 equivalents (respective to the amino acid) of DIPEA. Agitate the mixture vigorously for 30 to 60 minutes.
  3. To endcap any remaining reactive 2-chlorotrityl groups, add HPLC grade methanol, 0.8 mL per gram of resin, and mix for 15 minutes. Filter the resin and wash it three times with DCM, using approximately 10 mL per gram of resin. Wash the resin twice with DMF, twice with DCM, and three times with methanol. Dry the resin in vacuo. The substitution of the resin can be estimated from the weight gain. For an accurate measurement of the resin substitution, the amount of Fmoc released from a weighed sample of resin can be measured spectrophotometrically.

Removing the Fmoc Group

  1. To remove the Fmoc group from the first amino acid, suspend the resin in DCM/DMF/piperidine (1:1:2 by volume). Mix the suspension with a shaker for 30 minutes. Filter the resin and wash it twice with DMF.
  2. Test the resin for complete removal of the Fmoc group. Remove a small sample of resin and wash it twice with DCM to remove the DMF. Transfer the washed sample to a small test tube. Add approximately 100 microliters of 1:8 acetic acid/DCM (v/v). Let the sample stand for 30 minutes at room temperature. Evaporate the DCM and add one milliliter of 50% acetonitrile/water (v/v). Analyze the resulting solution by HPLC. There should not be any peak corresponding to the Fmoc-amino acid.
  3. If there is a significant Fmoc-amino acid peak, resuspend the resin in 1:1:2 DCM/DMF/piperidine and wait 30 minutes. Retest the resin. If necessary, repeat this process until Fmoc-amino acid is not detected.

2-Chlorotrityl Resin Cleavage

Acetic Acid Cleavage to Protected Peptides

  1. Prepare a 1:1:8 by volume mixture of acetic acid/TFE/DCM (approximately 20 mL per gram of resin). Suspend the peptide 2-chlorotrityl resin in half of this mixture. Allow the mixture to stand at room temperature for 30 minutes. Filter the resin and wash it with the remaining mixture.

  2. Combine the filtrates and add 15 times the volume hexane. Remove the solvent with a rotary evaporator. If the acetic acid is not completely removed, add hexane as needed and continue evaporating the solvent. The acetic acid will be removed as an azeotrope with hexane.

TFA Cleavage to Deprotected Peptides

  1. Remove the N-terminal Fmoc group using the procedure above. Wash the peptide 2-chlorotrityl resin three times with DCM to remove the DMF.

  2. Suspend the resin in 95% (v/v)TFA/DCM containing scavengers as required by the amino acid composition of the peptide. Allow the mixture to stand 30 minutes at room temperature.

  3. Filter the resin and wash it with a small amount of TFA. Combine the filtrates and add 8 to 10 fold cold ether to precipitate the peptide. If necessary, store the mixture at 4°C overnight to complete the precipitation.

  4. Filter the peptide using a fine sintered glass funnel and wash the peptide with cold ether.

  • Quantities and Prices

    To obtain a quotation for custom peptide synthesis, click HERE or contact AAPPTec by e-mail at sales@aapptec.com.
  • Contact Us

    United States Contact Information

    Thank you for visiting our website and for your interest in our company! AAPPTec has a dedicated team of chemists, engineers, technical consultants, sales representatives, and customer service representatives whom are waiting to hear from you. We look forward to speaking with you and working with you on all of your peptide-related needs.

    Address: 6309 Shepherdsville Road Louisville, KY 40228-1027

    Toll-Free Telephone: (888) 692-9111

    Telephone: (502) 968-2223

    Emails: info@aapptec.com, sales@aapptec.com

    Fax: (502) 968-3338

    Business Hours: Monday-Friday, 8am-6pm EST

    AAPPTec has representative all over the world. For contact information on the representative nearest you, please go to www.aapptec.com.

  • About Us

    AAPPTec, located in Louisville, KY, USA, offers custom peptides in quantities from mg to kg, in purities from crude to 98%, all at an affordable price. In addition to standard peptides, we also provide custom peptides with modifications such as cyclization, phosphorylation, acetylation, fluorescent labels, etc.

    Our Production Team has decades of experience in preparing high quality peptides, including long peptides, difficult peptides and peptides with special modifications.

    Our Customer Support Team has many years of experience in the peptide business and they are ready to help you with questions, orders, etc.

    AAPPTec continually strives to provide the latest technology, the most competitive prices and the highest standard of customer support to peptide chemists worldwide.

    We welcome your suggestions regarding our products and customer support. Please tell us how we can better serve you.

    Please send your suggestions to info@aapptec.com

  • What's News

    For upcoming shows, click HERE For the AAPPTec newletter Peptide News, click HERE For recent publications about peptides and proteins, click HERE.
  • FAQ

    1. How do I request a quote for my peptide?

    To request an instant quote, please sign up a free account with us and proceed to the peptide order page. If you do not have an account with us yet and would like to receive a quote for your peptide(s), please email us: www.aapptec.com or call us at 1-888-692-9111 and ask for a peptide service representative.

    2. What type of chemistry do you use?

    AAPPTec generally utilizes Fmoc chemistry for most peptide production.

    3. How do you generally purify your peptides?

    Our peptides are generally purified by preparative reversed phase HPLC, using trifluoroacetic acid (TFA) modified buffers at pH 2. Buffer A is 0.1 % TFA deionized water and buffer B is 0.1 % TFA in acetonitrile (ACN) at pH 2. Peptides are dissolved in either straight buffer A, or some amount of buffer B then diluted with buffer A.

    Sometimes it is necessary to use an organic polar solvent like (formic acid or acetic acid) in DMSO or DMF to aid in the dissolving of hydrophobic peptides but this is done on a case-by-case basis depending on the sequence analysis. The separation is monitored by UV at 214 nm and fractions are collected and analyzed by mass spectrometry for product identity and by reversed phase analytical HPLC for purity.

    The fractions are then lyophilized to remove the solvents. The fractions that meet the specifications of the order are then combined into one vial. Next, we run a final mass spectrum and analytical HPLC on the combined material.

AAPPTec offers Custom Peptides Catalog Peptides Immunology Polyclonal Antibodies Monoclonal Antibodies Amino Acids Synthesis Resins Coupling Reagents Bulk Amino Acids Bulk Synthesiz resins Bulk Coupling Reagents Peptide Synthesizers Freeze Dryers HPLC Systems HPLC Columns AAPPTec is your one source for peptides and peptide synthesis.